The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR merchandise were being digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was introduced by the short change https://haynesm666xjt8.wikiitemization.com/user